Biochemistry and Molecular Biology

I want to study science with the intention of being able to get pregnant and birth a child. Is Biology the field I should study? I have ideas of using either stem cell or gene therapy to be able to grow a uterus thus eliminating chance of immunorejection. Now the question is where do I get started? What materials do I need, where can I get them?

https://www.visualcapitalist.com/wp-content/uploads/2021/08/Biomass_v9.png ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: Would appreciate some comments on the above. “Surprisingly, at least to me, humans are relatively small” and my second query, what is the "uncertainty" in the given biomass numbers?

I’m completely stumped on how to use the Kd to gauge relative concentration of bound vs free protein. I was told something along these lines (I used "conc." instead of brackets because they weren't showing up properly): Kd = ( conc. A x conc. B ) / conc. AB "If concentration A is fixed and relatively low, and concentration of B is lower than Kd, then there will be relatively low AB and mostly free A." How does this make sense conceptually? I've tried to model it out, but I can't seem to get anywhere. Can anyone please help?

Hi I wish to isolate eggs from sheep faeces and subsequently extract DNA and run a PCR from them. Part of the egg isolation technique involves floating them on a dense liquid. The three commonly used floatation liquids are Zinc sulfate, saturated sugar and saturated salt solutions. I was wondering what are the effects of these substances on the performance of the PCR reactions ?

What is the Conspiracy theory of Inactivated Polio Vaccine?

Hi there, I have been recently been thinking about the determinant chromosomal positions we see for each chromosome within a given cell type, many of the chromosome pairs are quite far away inside the nucleus, and these positions seem to not be moving that much at all (that is, we can consistently find the same/similar topology in a given cell type). Now that is all good and nice, but it breaks my expectation of homology directed repair (HDR) a bit. In HDR, a double-stranded break on one chromosome is fixed through the recruitment of the other chromosome, and while I am aware that HDR is not as frequently employed within a cell as NHEJ (non homologous end joining), t…

What stops rocks humans rivers and atoms been the same ?

Does it differ for men and women?

So i´m supposed to find some genes that are able to replace an essential gene once they are overexpressed. Any ideas for that? Perhabs there is a method which i havent heard about yet? Would be very happy if anyone gave me some quality advice.😁

This might be a silly question but, does the body consume energy in the process of maintaining pH? I was reading about the so called "alkaline diet" and the misconception that different types of food changes the body's pH. I know that this is wildly untrue but, it made me wonder if there was a caloric benefit of eating alkaline or acidic foods. Can anyone more knowledgeable on the subject tell me if the body burn calories in the process of maintaining pH?

Some fungi and bacteria are capable of producing lignocellulosic biomass (like photosynthetic bacteria can produce sugars) just like plants, and I want to ask, if you know/think it is/might be possible to use them to generate large amounts of the product? To skip the growing of actual plants part.

Hi there, I have a question for you all. Sometime, after processing cord blood samples, the bc count is higher than the whole blood count. It happens with an average of 10% of the total samples I'm trying to understand how this can be possible, because obviously it is impossible to create cells. I'm using a sepax device and after the count is made with an horiba device. Some people told me that it could be either a problem during the blood mix or bc mix, but even with a right mix the problem persists. Thank you for your help.

Hi, When I perform qPCR the internal positive control of the reactions from 1-5 show low or no signals, compared to the rest of the reactions (approx. 20 reactions are performed in one run). PCR runs were performed by colleagues several times with the same material and everything went well. I am not sure what is going wrong and I dont know why the first reactions are affected and the other ones are fine, I assume either pipetting error (not enough IPC) or inhibition of IPC. I am thankful for any advice.

Hello, after analyzing exomes with AnnotSV I have some interesting duplications and deletions to validate. I only now how to validate deletions with qPCR and Sybr Green; However this is not the best approach for duplications. These duplicated/deleted genes also are not in ready MRC Holland MLPA probe sets. Which method is best for validating duplications and deletions of some exons in genes? Thank you!

Hi, anyone please make me clear if it is possible to extract DNA from bird feathers by magnetic bead-based DNA extraction method ? If any kind of literature or protocol is available on it then kindly share the link also. Thanks in advance.

Hello everyone, this is my first post and I am hoping it is the right place to ask. I just recently started cloning, PCR design and similar. I have very basic question and would like to get an answer step by step if possible, using an example 🙏🏻 So I have a pool of oligos (with short stretch of the same sequence at 5’ and 3’ so I can do PCR with it, for example to introduce nuclease sites). My question is, how do I design a primer for PCR to introduce biotin site and let’s say one of any restriction enzymes?? I hope I provided enough of info, I need only one primer. thanks a lot!!! 🤓

What are the main similarities and differences between a 310 helix and alpha helix. I know that a 310 helix has 3 resiudes per turn and an alpha helix has 3.6 but what other the other differences and simlarities between the 2?

Hi everyone, I want to simulate AMPK activation and I`ve been searching for reliable information about AMPK for a while now but most researcher differentiate on a large scale when it comes to Vmax and EC50/A0.5. My question is, if anyone knows a physiological possible score for those terms? I appreciate your help.

What is the difference between the action of antioxidant analysis in serum and tissue homogenates for example in liver? Like MDA,CAT,TAC and GSH and which is more accurate results in serum or in tissues homogenates?

http://www.sci-news.com/paleontology/bicellum-brasieri-09615.html Bicellum brasieri, a freshwater protist that lived nearly one billion years ago, had two distinct cell types and could be the earliest multicellular animal ever recorded. Found in the Scottish Highlands, the microfossil reveals a new insight into the transition of single-celled holozoans into more complex multicellular animals. “The origins of complex multicellularity and the origin of animals are considered two of the most important events in the history of life on Earth, our discovery sheds new light on both of these,” said Professor Charles Wellman, a researcher in the Department of Anim…

Why, in this method, when compared to "Spread plate", does it have less tendency of interference between one colony and another?

We all know that most heterotroph bacteria are not considered patogens, but why is it important for its density to be kept under control? If there is something wrong, sorry for my bad English. It is not my main language.

hi, guys do you think we can genetically modify a bacteria to repair our cells depending on tissue and this way to live thousand of years?

So, gram negative flagella have the L and P rings to help act as bushings in the LPS and peptidoglycan layers, respectively. Gram positive bacteria have a thick peptidoglycan layer, but no P ring to help the rod component rotate easily through it. I was just wondering if the biology of how it rotates despite the lack of a P ring is known- are there any known adaptions of the flagellum to allow this? Any elucidation to satisfy my curiosity would be nice.

There seems to be a discrepancy in what happens to CoA in the first step of the carnitine transport: 1. Some sources show, that CoA gets released in cytoplasm, while acyl-carnitine is transported to the intermembrane space 2. In other sources, acyl-CoA gets transported as a whole, and free CoA is left in intermembrane space. Does anyone know which version is correct? I've been trying to find out more about this, and the second option seems more supported, but what I don't understand is if CoA is released in intermembrane space, what happens to it afterwards? Is there a transport that moves it back to cytosol?