chanel_lee39

Thank you CharonY. Couldn't agree with you more. I have learned my lesson this time. I think more care really needs to be taken on this matter in our school.

Thank you all for the information. Yeah, my lab does have a lack of safety protection or education about this kind of stuff. My PI thinks that if everything is denatured, I should be safe. But I am just wondering can the virus somehow renature after thermo and detergent denaturation once they get in the body? Anybody knows?

Thank you. I will talk to the P.I. The viral vector is pLVX-Puro from clontech and it does not have any insert in it. I am not sure if any got into my eyes. And the samples were processed through SDS lysis, sonicated and denatured at 95 Celsius. They were in sample buffer when I splashed them, only a tiny bit got out onto my face. Is there a chance the virus could reform in this case?

Hi fellows, This sounds really stupid but I spilled some lentiviral proteins onto my face today when I was loading the proteins in the SDS-PAGE gel. I am not sure if they got into my eyes but I wiped them out immediately after spilling. And the amount was very small. The proteins were collected from 293 cells that were used to package lentivirus so they definitely have lentiviral particles in them. I lyzed them with NP40 lysis buffer, they were sonicated and of course denatured by boiling. Were these procedures enough to kill all the virus or am I still at risk of getting infected? I am just worried that is there any chance the viral particles could revive once they get in vivo? Thanks! Chanel