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gfritz

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    genetics

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  1. Good question. FRET probes can be used.on a CFX. FAM / HEX is fine. www.youtube.com/watch?v=4JBFpXVXhyw
  2. If the Tm is around 75C, it's primer dimer... compare to your NTCs. If it is higher in Tm, mis-priming due to poor primer design. Increase annealing Ta or redesign primers. www.youtube.com/watch?v=4JBFpXVXhyw
  3. Bored one day in the lab and found this funny video to entertain myself for 3 minutes. Who remembers those goofy infomercials?? www.youtube.com/watch?v=4JBFpXVXhyw
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