Hi there
I need some help with a PCR experiment I had to do for an assignment and I'm not sure if I completely understand whats happening in the agarose gel photo. I've added the gel photo and also the molecular weight marker used.
lanes (from left to right) 1=negative control, 2=chicken DNA, 3=cauliflower DNA, 4= Molecular weight marker
this experiment was to see if using the same primers produced from cauliflower beta-actin, and by adding them to chicken DNA if they would amplify the same sequence.
I would expect it not to amplify the same, and the gel photo shows the brightest chicken fragment higher than the cauliflower. but then there is also a fragment that is at the same height as the cauliflower but not nearly as bright.
I'm still trying to wrap my head around all this and not sure about what i'm actually seeing, if someone could help me on the right path that would be great!
Cheers
Ashlee
