tomsmithucd123

Imagine you want to do some functional tests on some resistant mutants. To aid you in this you use a set of old-fashioned F’ plasmids that carry various parts of the chromosome How could you construct a merodiploid strain that is heterozygous for your mutations (sequenced above)? Remember: every move you make requires a positive selection. How could you determine whether your mutation is dominant or recessive? What could you say about the protein whose alteration caused resistance?