johndohnal

Hi All, I'm looking for a PDB for 7α-hydroxylase CYP7A1 in complex with cholesterol. I've searched in the PDB database and I've found the files for the enzyme alone, and complexed with cholest-4-en-3-one and 7-ketocholesterol, but not complexed with cholesterol. This seems odd to me as cholesterol is its natural substrate. Is it possible that the PDB file doesn't exist, or am I missing something? Thanks! John

Hi All, I'd like to know if there are any known enzymes that cleave the sidechains (not the backbones) of lysine or arginine. Smaller enzymes would be better. If unknown for lysine or arginine, please let me know of any enzymes that will cleave the sidechains of any protein. Thanks, John