ateijeiras

i just have problems with DCs, probably they have a lot of RNAse activity and low amounts of mRNA as they are not very active. I just want to know if somebody have met the same problem with this cells or similar cells, perhaps of the immune system Thank you for your reply

I'm getting very low amounts of RNA when extracting it with TRIzol form under culture differenciated human dendritic cells. I usually don't get more than half a microgram. Could somebody help me or has have the same experience?