I've got this assignment and it states that we can't use any methods that involve creating a library of cDNA clones, but is based on RT-PCR (Reverse Transcription – Polymerase Chain Reaction).
The only thing we know is the mature protein sequence.
How do I design a primer that is specific to the mRNA?
We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.