Hi! my supervisor wants me to use exosomes in our model and I am trying to follow th methodof Nat. Protocols El-Andaloussi et al 2012 and Alvarez-Erviti Nat Biotech 2011 but I am not getting any silencing in the brain and I have just seen these comments in you tube and in this blog talking about fraud and saying that this papers should be retracted! who should I trust? does anybody know other protocol for brain delivery of siRNAs using exosomes? I am quite desperate! any advice would be greatly appreciated!
http://metamodern.com/2011/03/22/across-the-blood-brain-barrier-with-exosomes/
Cheers
