justskipee

Charon - We did about 4 films and they all had the same pattern, so it is reproducible. When we slightly overexposed it (example posted) we could see the lanes underneath. We have had successful attempts 4 months ago, so this has caught us off guard. MedGen - We placed the membrane with fuji film medical x-ray film in an autoradiography cassette. Here is a summary of the steps involved. Full instructions: http://www.roche-applied-science.com/pack-insert/2209136a.pdf When we place the membrane into the opened hybridization bag, we then put 40 drops of sustrate solution. We didn't see any excess pooling or anything that would correspond to the "river" looking pattern. Do the assay instructions above help at all? Thanks for your help!

Hello there, We are using the Roche TeloTAGGG Telomere Length Assay to measure telomere lenth in peripheral blood mononucleocytes. (manual is found at https://www.roche-applied-science.com/pack-insert/2209136a.pdf). It is a non-radioactive chemiluminescent assay, that involves digestions, gel electrophoresis, telomere probing... We have been having a very weird problem. Our film shows nice smears for each lane, but it is like a small river flowed across the lanes. No one has a clue what would cause it, and it has been very frusterating given the cost and time we have used already. Here is a link to a picture of our film. Does anyone have any clue what could be causing this problem???? Thanks so much for your help, Justin