Hello, I have ordered primer set according to the source article, as well as several other articles, should yield a 740 bp PCR products, but when I performed the protocol according to them I get 500 bp PCR products bands when run in gel. When I searched about that I have found another article where they have gotten 600 bp PCR products using the same primer set.
The articles with 740 bp:
http://www.apocpcont...emant K Bid.pdf
http://biomed.cas.cz...5/zajickova.pdf
The article with 600 bp:http://www.lifescien...802_120_131.pdf
My PCR products and ladder information are the two attached images
What do you suggest I do to make sure if my PCR product is right and how will I know the expected band sizes from the restriction digestion with Taq I?
My primers are:
Forward
5’-CAGAGCATGGACAGGGAGCAA -3’
Reverse
5’-CACTTCGAGCACAAGGGGCGTTAGC -3’
