LauraW

I think technically, any oxidising agent would cleave off the glycine side chain

Hey! So, I'm after reading a tirade of experimental papers and journal reviews for a college assignment and all of them seem to glorify their subject molecule as either "crucial", "extremely important" or "invaluable". For a change, instead of hearing about vital, couldnt-do-without proteins and PTM's... I'd like to hear about a completely dispensable and redundant protein. What do you think is the least important human biomolecule? I vote for one of the many olfactory receptors we have.

Hey! I don't quite understand the wording of the first question but I think I get the second question. Apparently, the optimal vector:insert ratio is 1:3. Your answer can be determined by the following equation: ng of vector X kb size of insert insert ------------------------------------------ x molar ratio of ------------- kb size of vector vector Plug in your known values: Kb size of vector: 4.9 Kb of insert: 0.1 (100b not Kb!) ng of vector : 50 molar ratio of insert: 3 molar ratio of vector: 1 Throw these into that equation and bobs your uncle! (not sure why they gave you the MW of dsDNA or ssDNA) I hope this is right.