Tushare

I am working with dissociated neurons and I would like to figure out a way to examine a post synaptic cell during perturbation of cellular and even sub-cellular pH (at the spine heads), do you have any specific metabolic enzymes that you would suggest? Inhibition of metabolism is very destructive and very pathological, I wondering about over expression of a poly histidine or glutamic acid or aspartic acid, I'd ideally like to drop the pH from a normal 7.2-7.4 down closer to 6.8-6.5. Throwing on antimycin A has worked well in the past over an hour with my neurons but this affects both neurons at a synapse within my dish. Maybe a dominant negative of oxidative phosphorylation? I'm not sure what the effect of blocking this will be over a 24hrs period.

Hey all I would like to find a way to manipulate the intracellular pH of a cell without affect all the cells in my culture. I know I can use a number of approaches to acidify of alkalinize my culture be I was hoping to find a method that would involve the transfection of something genetically encoded. This would give me modulation of intracellular pH in a small percentage of my cells in my culture. Any thoughts would be much appreciated. Cheers,