BarbaraBB

I am collecting evidence, even anecdotal, how does single nucleotide deletion or insertion in primer region affect the outcome of real-time PCR. I am most interested in how much there is a delay in Cq (treshold cycle value) if compared to perfectly matched primer. But if you do not have Cq data, I also collect "Yes or No" answers, saying, whether there was amplification or no amplification. I am interested specifically in insertion or deletion mismatches. (For substitution mismatches, there is a lot of data.) I will appreciate any published or unpublished evidence.