bashert

hi! Yeah, Chad, some of the other proteins are expressed at higher amounts. Thanks Charon, I'm redoing to see if I can repeat and try to be exact with my normalizations. The more I think about it, the more it seems likely. thanks all!!

hi CharonY...yeah, by background I mean the other normally expressed proteins produced. I normalized by cell paste concentration prior to lysing the cells.

I've measured expression by protein. I'm currently trying to find examples of silent mutations in one protein that improve global protein expression. I think that is basically what I'm seeing.

hi everyone... I'm hoping to get a few ideas about what could be going on with this expression system. I'm currently expressing an enzyme in W3110 cells. We discovered a silent mutation that increases production of the enzyme of interest but it also seems to increase the background expression as well. Any thoughts on if that could be possible? Or is it likely to be an artifact of the experiment? thanks~~~!