jwhit117

Good day! I am a 4th year Honour's student at the University of Ottawa as part of the Fortin lab group. This is my first post to this forum and I thank you for reading my post - and hopefully fielding it as well! At the present time, I am currently conducting some Geochemistry work as part of my cross-disciplinary Honour's project but, as a biomedical and medicinal chemist by training, I have not had any formal practice in programs such as PHREEQC. From my research online, it is clear that this may be a forum where I can get an answer to my problem! I believe my situation may be a simple one to comment on and fix. As a complete novice to the program I have not written any specific code to review. However, I understand how to enter and define basic solutions from my online research. My colleagues also only have a basic understanding of PHREEQC as it is related to geochemistry and suggested that I get into contact with leading experts knowledgeable in the field of geochemistry. So I am here! At present, I am attempting to model a media solution containing the following compounds: (1) Trizma Base (10g/L) (2) Urea (0.125M) (3) (NH4)2SO4 (0.005M) (4) Yeast Cell Autolysate (10g/L) (5) Trace Metal Addition (Calcium, Magnesium, Zinc, Copper, Iron, Nickel) pH = 8.0 Temperature = 28 degrees celcius My question is: How I would input and model compounds such as Trizma base (2-Amino-2-(hydroxymethyl)-1,3-propanediol), Urea ( (NH3)2CO) as well as amino acids (the Yeast Cell Autolysate being a heterogeneous of phosphate, trace vitamins and surplus amino acid content) into the program? Most especially the amino acids with their reactive ammonium and carboxylic moieties! The penultimate goal is to determine if any of the trace metals I am adding into the media will precipitate, thermodynamically, at the concentrations I intend to add to the media solution (0.01 - 0.20uM). The addition of the trace metals is to see their effect on the growth of a bacterial strain I am working with and if the incorporation of the metals (tested one at a time and in different bacterial flasks and batches) affects the activity of a special enzyme (urease). Thank you for your time and effort in fielding this question, if possible! I hope that the parameters are clear and that an answer could be fielded with little inconvenience or strain on anyone's schedule. Please know that anyone's time and effort are certainly most appreciated! With kindest regards, Jwhit117

Good day! I am a 4th year Honour's student at the University of Ottawa as part of the Fortin lab group. This is my first post to this forum and I thank you for reading my post - and hopefully fielding it as well! At the present time, I am currently conducting some Geochemistry work as part of my cross-disciplinary Honour's project but, as a biomedical and medicinal chemist by training, I have not had any formal practice in programs such as PHREEQC. From my research online, it is clear that this may be a forum where I can get an answer to my problem! I believe my situation may be a simple one to comment on and fix. As a complete novice to the program I have not written any specific code to review. However, I understand how to enter and define basic solutions from my online research. My colleagues also only have a basic understanding of PHREEQC as it is related to geochemistry and suggested that I get into contact with leading experts knowledgeable in the field of geochemistry. So I am here! At present, I am attempting to model a media solution containing the following compounds: (1) Trizma Base (10g/L) (2) Urea (0.125M) (3) (NH4)2SO4 (0.005M) (4) Yeast Cell Autolysate (10g/L) (5) Trace Metal Addition (Calcium, Magnesium, Zinc, Copper, Iron, Nickel) pH = 8.0 Temperature = 28 degrees celcius My question is: How I would input and model compounds such as Trizma base (2-Amino-2-(hydroxymethyl)-1,3-propanediol), Urea ( (NH3)2CO) as well as amino acids (the Yeast Cell Autolysate being a heterogeneous of phosphate, trace vitamins and surplus amino acid content) into the program? Most especially the amino acids with their reactive ammonium and carboxylic moieties! The penultimate goal is to determine if any of the trace metals I am adding into the media will precipitate, thermodynamically, at the concentrations I intend to add to the media solution (0.01 - 0.20uM). The addition of the trace metals is to see their effect on the growth of a bacterial strain I am working with and if the incorporation of the metals (tested one at a time and in different bacterial flasks and batches) affects the activity of a special enzyme (urease). Thank you for your time and effort in fielding this question, if possible! I hope that the parameters are clear and that an answer could be fielded with little inconvenience or strain on anyone's schedule. Please know that anyone's time and effort are certainly most appreciated! With kindest regards, Jwhit117