GreyScott20

Hey everyone, Just did an experiment spectrophotometer looking at the effects of high temperatures and found something rather unusual. Its with the secondary azo dye; congo red with beta-glucans in the form of brewers yeast and oyster mushroom tablets BUT at temperatures of 50 and 70 degrees celsius. Repeated tries over 3 times and found that the higher the concentration of the tablets, the lower the absorbance read by the spectrophotoemeter. Essentially saying the presence of these dye complexes actually decrease absorbance. Can anyone help me out and give me a hypothetical or actual reason on why this happens please? Literature and Journals aren't really explaining this. But if more details are needed ont he experiemnt protocol or certain conditions, I'd be happy to co-operate. Thank you very much all, Scott.