thomasjohn497

In my biochemical engineering book, in the section regarding cellular oxygen transfer rate from the media to the cells, the authors introduce three equations (see the attached photo for equations). In presenting these equations, the book states that, "this is the most accurate way to measure the mass transfer coefficient (kLa) in a fermenter." However, the authors do not explain in the book how to actually go about measuring the kLa using these equations. I was wondering if anyone with knowledge on this subject could provide further information regarding the determination of kLa using these equations, it would be a big help.

In the production of SCP involving yeast fermentation, I was wondering why the media needed phosphate? Why is it essential? Does anyone know the pathway that the phosphate enters once it is taken up by the cells? Also, metabolically, what is the difference between KH2PO4 and Na2HPO4? I have noticed that both compounds are added to the media, usually more KH2PO4 than Na2HPO4. Are both required for the successful production of SCP? Or could the organism ferment well with only KH2PO4? Thank you for your responses.