jvdb

Dear all, I have a question about the use of mutant backgrounds in Arabidopsis. Just to confirm my thoughts. Mutant backgrounds are used to search for even more aberrant phenotypes. New i wondered, if you insert an extra mutation by for instance EMS mutagenesis in a certain mutant background, and you self your plants (because you want your newly inserted mutation to become homozygous), does your original mutated background remain homozygous? I thought it did, but i started to doubt.. Thx in advance!