For my biology lab, we had to find the pH and its effect on amylase activity.
I used 1mL of acidic/basic/neutral starch solution and 3mL of 5% amylase solution.
After combining the two solutions, I waited 50 minutes for the reaction to occur.
(We cannot change the temperature. Therefore, room temperature)
After 50 minutes, I used the Benedict's reagent and heated it to see the colour change in the solution.
However, I did not observe any colour change. My hypothesis was that the neutral solution will have more
monosaccharides for its product! However, when I did Lugol's test (iodine), all three solutions tested that there is no
starch in the solution. Does that mean that the product is a disaccharide? I am confused with the results.
How should I modify my lab in order to see changes through the Benedict's reagent, not Lugol's reagent?
Thank you in advance!