GeneMan

Thanks Charon Y and FiveWorlds Im good for electrophoresis, thanks Charon Y I think FiveWorlds is on to some thing here. My method actually works very well with acetone, its just huge due to the volumes needed after atting the DTT solution to adequately remove the cells (happy to supply FiveWorld, although is in the lab boom atm so will need to transpose to doc.) Hence when you add the acetone for the correct volume to voluem ratio, it gets volumous and and difficult to manage a range of samples/patients. Trying to process 10's of snots. Ideally I want a kit, eg there are saliva kits, coz 1. we can afford it, and 2. my time is costly (although I suspect the kit is worth more than my time (sigh)). Chees for the advice though people. GeneMan

Hi all Anyone out there know how to isolate total protein from respiratory mucus, namely sinus snot? I have a long method that removes cells (dint want them in there, just mucus protein!), using DTT, followed by an acetone precipitation. Its way labor intensive and uses allot of acetone to work. I want a kit or a nice short method that takes into accout removing cells. Anything??? Cheers GeneMan