incub0x

I appreciate your comment, I will try, thank you very much

I am using milliQ water. It is an 1.5% agarose gel and Is is a 100bp ladder

Hi: I am a PhD student, I am trying to get Whole Human Genome Restriction with Mfl I. My conditions are: 10x L Buffer-------2 µl DNAg ---------------1 microgr Water deionized----up 20 µl Mfl I-----------------1 µl (10u/µl) 37º C--------18 hrs 65º C--------15 minutes But I have not obtained restriction of the genome. For that reason I am consulting you about this. Maybe I am doing something wrong or I need to do something more. I appreciate your advices. Thank you.