Hi everyone, I´m on a Master program and next week I have to go to a Biotec company to solve a problem in just 1 week... I´ve been asked to characterise the human epidermal growth factor at least with two proteases. This protein is very small around 6kDa, and I´ve never done a peptide map of any protein. If anyone could guide me through the basics would be great. I need to understand what should I know about the protein and how to select the appropriate proteases, and how to actually see in a simple experiment the peptide map, like in electrophoresis.
Thank you.
Alvaro
