SpellForce

Vaccine has been done, but it is not a 100% prevention of having or transmitting it... I am careful and I follow protocols, I just wanted to hear if anyone had experience with this bacteria etc.

Hi, I am curious. How dangerous is to work with Bordetella pertussis? For example, I am very careful and most of the work is under the hood (except for centrifugation and spectrophotometry). But then, I need to take a small quantity of cell dispersion and carry it through the lab on a glass slide for microscopy. Is there any possibility that bacteria go into air or otherwise endanger me? Thank you.

Hi, I have a question considering a swarming assay on E.coli... I did a soft agar (0.3%), in LB, and placed a drop of bacteria in the middle of a plate. But after 24h, they just grew in a small radius, like in the photo: DO you think I have non-motile E.coli? Cause I would expect a bigger circle. Or Im just doing something wrong... How is this different from a swimming assay?

Hi everyone! I would like to ask for some advice concerning the work in P2 laboratory. It includes work with E. coli, Staphylo and Streptococcus. I always disinfect workplace with 70% ethanol. I also clean the floor from time to time (not really often), with some detergent. However, I am mostly concerned about airborne contamination. 1) Is it possible to breath in some bacteria when I open the ependorf containing their suspension (mentioned bacteria)? 2) Should I put a mask when I open the incubator, since I feel its humidity and warmth (when taking and placing cultures)? 3) Why is it not advised to put things on the floor? Can bacteria travel on the floor and climb up the things? 4) Even though I wear gloves, is it possible to have bacteria on hands? 5) Can bacteria actually survive on any object or body part, if its dry and without nourishing media? Thank you so much for your help and advice. Cheers.