Adriana Reis

yep i believe we are being observed

Hello Everyone I am asking for a word of advice. I am supposed to be doing fermentations soon. My problem is I never really had much experiences with these. I need to run 3 fermentations like this. 1- Yeast DSM 1333 s cerevisiae 2- Yeast + Gram positive contaminant (Lactobacillus fermentum) 3- Yeast + Gram negative contaminant (E.coli) I decided to use these conditions for 3 fermentations Broth= Water 1L, yeast extract 10g/L, glucose 20g/L, peptone 20g/L pH = 6 temperature 30C 160rpm For starter cultures and yeast i used hemocytometer and the formula initial concentration x volume i want to know = final concentration (start fermentation) x volume of fermentation (250mL). so ill find what volume i should pour in to start fermentation but i find very hard to count bacteria in hemocytomer. can I use OD600 to calculate the volume i need to pour of bacteria cultures> if so how do I do it? my bacteria (starter culture) ended growth in OD=2 can i dilute it? and sayOD = 1 = 10^7 cfu/ml? Thank you for your time Adriana

hi Im adriana. im a microbiologist. portuguese living and working in UK. need more friends. even if computerized friends.

so many the ones i remember now maybe Jane Eyre,

Finishing Preacher and started Ten Grand. AMazing comics I recommend Batman Hush and The pride of Baghdad.

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my limbs go numb lots of times specially if I stay in same position for like 4min or more. i guess theres something wrong with me. in fact i thought i had MS i have tingling, pins and needles or numbness, muscle weakness, very pronounced reflexes, muscle spasms, or difficulty in moving; difficulties with coordination ; problems with speech or swallowing, double vision, feeling tired, etc etc but i was told i am crazy and imagining things. so far im still the same as 3 years ago... only my tinitus has worsened...

never heard of this before. it is rather interesting. thanks for giving me new insight

just media scare want to worry about a disease? worry bout HIV, TB, cancer, Influenza, malaria, and so many others that kill more than the ones Ebola outbreaks together have

try and see how it goes. if after a couple days you still havent improved then yes go to doctors and they get you antibiotics