Albert-LV

ooookay here, I know it was urgent, but I figured out I'd post an answer because this page shows up in google when looking for LH-PCR... you made several mistake : first you amplified the full length gene, you'd better use the variable V3 region (Ghiglione, 2005) : then, from what you say, it seems you ran your PCR fragments on an agarose gel (or did u use a PAGE ?), you should run the fragments on a capillary systems, it's more accurate (no, it's required).