I have obtained concentrations for 3 unknown proteins through BCA assay. I ran kinetics on a spectrophotometer for each unknown at two different different volumes(5uL and 10uL). We only did 1 trial for each volume for each unknown(6 measurements). We ran out of time to do more and we were having some issues with the substrates. Regardless, I now have to write a report on the experiment.
This was a citrate synthase experiment. I have no idea what I am looking for. What I do know is that when we increase the volume of the protein samples the ABS/min goes up. Im assuming I should solve for Km and Vmax, but other than that I have no idea what this means as it relates to citrate synthase. Ive asked the instructor and am told to interpret it myself. I am lost here. Help please.
