Hello, I'm currently Studying EPO gene expression. I have read a few papers on analysing EPO receptors through leukocyte extraction and running an ELISA on them. I have been told that I can analyse the gene expression of EPO through leukocyte extraction also, and then running real time PCR and then a high resolution melt to detect a change from my intervention. Although I can not find any papers that replicate this through leukocyte analysis. Please could someone tell me if this is correct and if so direct me to some relevant papers that look at gene expression of EPO from leukocytes through PCR methods/protocols. I'm new to this area so if any of my explanation is confusing/wrong please let me know. Thank you
