I need a basic layout on how to go about answering this type of question.
You have available an Hfr lac- ala+ vitB+ tetS strain of E. coli and an F- lac+ ala- vitB- tetS strain.
You also have a P1 transducing phage and a tube of plasmids with a gene for tetracycline resistance. These are the only supplies you can use ( besides media and reagents). Describe how you can isolate a pure strain of E . coli lac+ ala + vitB+ tetR, where all genes of interest are present in the chromosome of the bacterium. Include gene transfer steps you would use, as well as the media ( m9 or Lb) you would use to select for successful gene transfers
Would appreciate any help as soon as possible, thanks
