kixxer

well, I´m very sceptic about these HIV-vaccines. I guess the money spent on the development of vaccines would have better use in other areas of disease control, etc. informational work in Africa. I´ve been somewhat involved in HIV vaccine development at the university I work at, and until now I at least am not convinced by the vaccine trials currently in testing.

we use LB agar only for standard culture, and it works fine

hi, I´ve had some problems with getting my E.coli expressed proteins endotoxin-free. There are commercial Kits with polymyxin- colums around, but when I use them not only the LPS, but the protein as well, gets bound to the column and I loose all my proteins. HAs anybody done this? greets, kix ________________________ http://www.biologia.fi

I´m sorry, but I didn´t understand your question there ? Why do you want to put the housekeeping protein on the SDS-Gel? Just as positive control? Or are you trying to do some shift-assays? greets, kix ____________________ http://www.biologia.fi

Yes' date=' I guess you have to if you want to work as a scientist;). But it hasn´t to be Medicine you study, you can do biology as well for example. I even know chemists who work in HIV-vaccine development and do pure molecular biology. Difficult to say..usually (at least in Europe) they tell you what they want to do with the money, etc. help children with leukemia. In these cases I guess they just try to make the patients life more worthwile, cancer treatment isn´t always that cheap. Some people have to continue to take hormone-pills for their whole life after successfull cancer operation, and those pills do cost a lot! Still there is a constant flow of financial aids to research. Lots of research funding comes from the state or government or private institutions. greets, kix _________________________ http://www.biologia.fi

In Germany your PhD-thesis in biology for example takes approx. 3-5 years. Sadly, there is no way of getting a job both in research or industry without your PhD, so 95% of all people with masters degree continue with their thesis. greets, kixxer ______________ http://www.biologia.fi

hi, just wanted to ask if anybody here has been working with Parvovirus B19? I myself am very interested about this very small, relatively unknown virus which seems to be associated with a very broad range of different symptoms and diseases in humans. greets kixxer __________________ http://www.biologia.fi

...i´ve done mine at a institute for med. microbiology, chair of virology, though officially it was counted to genetics. But it was pure virology and immunology to me;) greets, kixxer _____________ http://www.biologia.fi

hmm...how many sections do you want to have under "Biological sciences"? I mean, the usual thing I guess is to do the differentiation in the basic fields of research, e.g. zoology, botany, microbiology, genetics, biochemistry etc. If you want something more general, then maybe the first step would be to divide the links into molecular and organismic categories. I´m not quite sure I got your question right anyway, so I hope this isn´t complete nonsense I´m writing here;) greets, kixxer ____________________ http://www.biologia.fi

it means, that the two genes or gene-fragments you are going to fuse do not undergo frameshift, that means that the amino-acid of the two components does not change. greets, kixxer ___________________ http://www.biologia.fi

well, don´t take my word for it, but the way I understand it, the dilution with water and dye reagent do not matter in the measurement, because you use the same amounts of them on both the standard and the sample. So both OD´s you get are relative to each other. The reason why you dilute the samples anyway is to get to a OD-region which is nicely measurable, if there is a too high protein concentration in the tube, the test will produce wrong numbers. In the end all you do is compare the OD you got from your sample with the one of your standard. And you only can compare them if they are equally diluted. But as I told you, I´m not the expert on this stuff either:(., so this stuff might be horrible wrong too. Some experts help here please? you can make a Chart with your datapoints (OD´s of the standard) and then let Excel add a trendline to the chart. For this, you need to right-click on the graph and choose "add trendline --> linear". I hope I could help you a bit, greets, kixxer ________________ http://www.biologia.fi

yes, you basically could, like mentioned already, but in that case I think the immune system would recognize your "modified cells" because of the viral proteins and would destroy them. This way you would induce a severe autoimmune response and cause more harm than than the virus itself in a cell (at least in some cases). I hope I´m correct here? regards, kix __________________ http://www.biologia.fi