tmx3

Last I checked, it was sunny and you're with your kids. What business do you have writing to me now? And here's the last message you get. I'm deleting my account with this useless, worthless site full of pompous arrogant pseudointellectuals like yourself who want to belittle the opinions of others who come from a perspective of faith, who have genuine interest in helping others, all because you think you're "smarter than thou". Please, take a seat. Your knowledge came from your teachers who settled to get paid $15/hour to support their families if it means feeding minds like yours bullshit theory after another bullshit theory. And you'd believe it. But come to the idea of God, and everybody wants to act like we're talking about santa claus. I'm done disrespecting myself by entertaining this bullshit argument with you. I call you out on your lack of civility. Now you want to wear a mask and fake being civil. Get out of here

Nice acting you do. Here's some advice. The next time you want to belittle someone's genuine opinions when they sought to help OP who seemed to be having an existential crisis, do not come across as an arrogant, self righteous know it all. Don't "all the best" me with your fake self. Humble your arrogant ass self.

Okay. So the rat running away from the cat proves free will. And the parasite inhibiting the rat from running away proves the parasite inhibits the rat's free will. And, even using your example... if a gun is pointed at someone, their will is manipulated by the gunman. They give up money, not wanting to--doing something against their will. In some cases, when given the chance, one would fight back. In BOTH cases, free will is exhibited--one, where it is imposed upon and limited, and the other in which it is acted upon. I quoted him--it's directly to him. Open for all, yes, but directed towards him. And I welcome all comments--just lose the attitude. LOL. I recommend you try harder to prove me wrong. You can't, keyboard warrior. You just want to sit there and act like you have a point, but you don't. Funny how when I tell you to stop throwing words you learned in writing class at me, you have a lot less to say.

You pointing a gun at me to take my money is an example of you imposing your will and LIMITING MY FREE WILL by making me do something I would otherwise choose to not do (unless of course you were homeless or needed the money, then I would). A better example? Maybe. But it's not the only way of showing free will exists. Then again, this could be another scenario.

A parasite making the rat do something that it would otherwise choose to not do, is an example of free will on the rat's part. It's that simple. @zapatos

My message is for OP. Not you. Keep your attitude to a zero or don't bother messaging me with your disgusting self. You're superior to no one for you to go on typing on a keyboard like you have all the answers. I'm giving my input and perspective to OP. Allow me to do that without you coming at me like a disgusting, attitude filled narcissistic pretentious know it all. The fact that there is absence of free will, means there is free will to begin with. Try to use common sense and not a whole bunch of words you learned from your writing class that you want to throw at me, okay? Okay. That doesn't even make sense. Who in their right mind would even want to put themselves into harm's way? Inherently, we want to do the right thing, the thing healthiest for us, be at peace and in harmony with other beings. Why would anyone willingly choose some disaster to befall them? Clearly it was the neuroparasite manipulating the rat's will to do itself any good, by forcing it to go into a situation where it would do itself harm (get killed, get eaten). Its free will is being affected. Its will is being imposed upon by the will of another. That is the whole point.

I completely believe we have free will. I would give an example from the Holy Bible but refrained since I'm not sure if that's the kind of evidence you'd want to hear. Let me know if you do, and I'll give you my perspective on it. From a more scientific point of view, you'll find this interesting... Look up this disease called Toxoplasma gondii, research it a little. You'll find that it's a parasite wanting to live and reproduce in the intestines/stomach of cats but can only enter the cat through an intermediary host (like a bird or rat) as it needs an intermediary to morph into the form which can then travel within a cat's body. For a while, people noticed a strange type of "bravery" occurring in rats, where these rats would jump at cats instead of running away from them. When these rats were taken into the lab for testing, they were found to have this same parasite living in them, and these parasites were essentially manipulating the rat's normal behavior (which would be to run away from cats) and instead were a cause for the rats to be appearing brave and jumping toward the cats--basically doing what they would otherwise choose to not do. The goal of this neuroparasite is to have its intermediary host ingested by the cat, so that the parasites can then make their way to the stomach/intestines of the cat, which is where they reproduce. Look at it this way... a parasite was able to alter the way a rat would normally behave, so that the rat would specifically have itself killed and eaten by a cat, which is where the parasite was trying to go... It still blows my mind thinking about it, but, yes, free will does exist. That's half my take on it; the other half I'm leaving out to avoid the type of talk people these days run from.

@Strange @studiot thanks I'll see if it helps

@hypervalent_iodine I see. But my question is, why is matter considered particulate rather than elemental? And how does ratios of matter disprove elementalism, if matter is of that element? For example... CO is gas, a type of air. That (being gaseous) is its element (air). How does comparing the ratio of oxygen to carbon in first carbon monoxide and then carbon dioxide, and then making a ratio out of those two ratios, support the idea of the particulate nature of matter versus the elemental nature of it? What does that have to do with elementalism? Isn't matter both particulate and elemental? I'm asking from a philosophical perspective. Why can we no longer say that matter is elemental? Why do we have to agree with a "particulate" or particle-nature view of matter?

@hypervalent_iodine I'm talking about Aristotle's elementalism.

This is a topic that I've been thinking about, but before I ask my question, I just want to give some background info.: In 1804, John Dalton published his law of multiple proportions, which states: When two elements (call them A and B) form two different compounds the masses of element B that combine with 1 gram of element A can be expressed as a ratio of small whole numbers. So, carbon monoxide, CO, has a ratio of 1.33 when we divide the mass of Oxygen with 1 gram of Carbon (Carbon equalling 12.01 g or 12.01 amu in 1g of Carbon). And, carbon dioxide, CO2 has a ratio of 2.66 when we divide the mass of Oxygen with 1 gram of Carbon (again, Carbon equalling 12.01 g or 12.01 amu in 1g of Carbon). The ratio of these two...ratios?...will give a small, whole number: 2.66 / 1.33 = 2 Dalton was able to overcome a 2000-year-old perspective (elementalism) and push his view that matter is particulate instead of elemental by using the weights of samples of matter, and by demonstrating that matter pairs up in ratios. Elementalism implies that, basically, matter of one nature or type is different than that of another...so, think: air, fire, water, earth... That sort of thing (not really sure how to explain it). My thinking is this... carbon monoxide and carbon dioxide are two different gases. From an elemental perspective, they are different in nature (one is more poisonous than the other), though they are both gases. And, even though they are different than each other, they are still gases...and they are still of an "air" element or type. So, here is the question... Why is it that comparing 1g of an element to another unknown amount, is a determinant for whether or not matter is particulate in nature instead of elemental? I mean, if you take 1g of whatever, and then keep adding more and more of another type of whatever, won't it be just a whole lot of whatevers trying to bond with each other? What if you take 1g Carbon and then oversaturate it with Oxygen? Different types of gases will form, won't they? If different types of gases continue to form, how is that not indicative of the elemental view of matter? The way I see it...Dalton just published a paper about how elements combine in ratios, and that that is more than enough proof that matter is particulate instead of elemental. But, the way I see it, we're just observing different kinds of matter in the sense that, when we look at gases we're observing some different type of gas but of an air element, or when we look at minerals or salts or metals we're just looking at different earth-like elements... And I really don't understand the significance of ratios here. I mean, how does a ratio prove something like the nature of a substance, and furthermore, how does it prove that matter is not elemental?

Thanks for your question. Just as this is your first gel electrophoresis of PCR, this is my first time reflecting on PCR in quite a few years, so don't take my word for it completely but I'm going based on memory here to try to help you/answer your question since I see no one else has yet. Hopefully someone else chimes in and corrects me where I'm wrong. 1.- The impression I'm getting from your professor, since she's asking you to do them separately, is that she wants you to retain how to make the mix and also she wants you to be careful/not wasteful of the products. It's a tedious process, and the best way to learn is by repetition. The fact that your professor's asking you to repetitively go through the protocol shows me she really wants you to learn and perfect your method of PCR. More importantly, I think your professor doesn't want you to affect the quality of the outcome of PCR. Perhaps making a larger batch could cause you to dilute the primers more than what they should be, which with one mistake the whole batch would be ruined. From what I recall (years ago), primers are expensive so she also probably doesn't want you to waste the whole thing in one go. Instead, if you work on them one at a time, it'll help for keeping the outcome consistent with minimal/negligent errors. So, although I don't think it's a necessary measure, I do feel it would be the smarter thing to do if you really want to ingrain PCR technique and also take precautionary measures as well as keep the outcome consistent. Also, it could be the case that one of the primers may require a different step which cannot be applied to other primers. 2.- Primer = DNA piece that will be basically copied/recreated. DNA is molecular compound which means having the DNA of one species of bell pepper should be similar to another species of bell pepper. This means the environment in which DNA reproduces should be the same. So, no I don't believe each primer will have temperatures different than one another's but perhaps there may be additional steps required to produce a certain result you're looking for (one of the species). 3.- Blank is supposed to stain so that you have an independent/standard component to compare the rest of your strands with. The ones that didn't show either weren't dyed or something may have gone wrong – most probably a forgotten ingredient (which is probably the reason why your professor wants you to repetitively make the batches), or something may have gone wrong. This link may help with that: https://www.fws.gov/aah/PDF/PCRTS1_NoBand.pdf Try troubleshooting using that. Hope this helps!

Chess. Lol. Wanted something short and sweet to login and play chess online with people. Yup.

College is where you go for undergraduate studies in a certain concentration of a subject (examples of studies that you would major in, in a college are: anthropology, biology, chemistry, math, physics, english, linguistics...just google any college/university and search through “undergraduate programs”). Graduate programs are an even more specific course of studies that you undergo after completing a bachelors degree at a college/university. MCAT is an exam taken for medical schools. GRE is an exam taken for graduate schools. Are you looking to find a way into medical school or graduate school?