SteveF008

Okay let me get away from bacteria and use two organisms with an 18s rRNA sequence Using a diatom sequence Phaeodactylum tricornutum genomic DNA containing 18S rRNA gene, strain M17 compared to sponges gets this reult; 97431TCR013, a total score of 1775 with 87% identity, considering the e-value is 0, would you consider this a very significant alignment? Thanks

The main question was why that bacteria was aligning better with the sponge than with other bacteria, but I would appreciate guidance on isolating bacterial strains from sponge genomes, is that pretty straight forward or something that would take a lot of time explaining? I know how to find the sponge genomes, does this require primers?

I put up an updated BLAST result link, here it is again 96TDR7FH01R. I apologize for my ignorance here but I'm taught at using this tool, in fact I'm self taught in general on this subject, but I try. It's an 18s gene I thought it would be 16s. Here is the hit Azotobacter vinelandii isolate DNA101014 18S ribosomal RNA gene, partial sequence

Updated result link 96TDR7FH01R

I'm looking for a little help or just confirmation on whether or not the results I'm getting from BLAST mean anything or if I am completely not usiing this tool properly. I am using a sponge sequence Geodia neptuni 18s ribosomal RNA gene, complete sequence GI = 53771873 and running a nucleotide blast against all bacteria and get this result; 92TUW20U01R A score on the alignment of 1459 and 82% identity with the bacterial sequence identified as Azotobacter vinelandii is far better of an alignment than blasting that bacteria against all other bacteria. That doesn't seem right to me.