lothloran

The purpose of the project is to preserve my genetic material and not have to pay thousands of dollars over the years to a sperm bank. I have all the necessary equipment, the LN2 and C2H6O2, the dewar, and access to as much liquid nitrogen as I would ever need.

I see how you've achieved nearly 8000 posts. Do you have an answer to the question or are you just going to continue being pedantic?

Hello forum. I've recently decided to store my genetic material in case anything should happen to me, the problem is I do not have the money to store it in a sperm bank, but I have ready access to liquid nitrogen and can whip up ethyene glycol fairly easily as a cryoprotectant, The process itself is fairly straightforward and I'm confident that I can achieve effective results with a bit of trial and error. My question is, when it comes to the dethawing process, I understand I need to wash out the cryoprotectant with a culture medium then separating the culture / glycol with a centrifuge. I'm not sure which culture medium would be the safest to work with and I am not sure whether a 4000-5000 RPM centrifuge would be effective or if I need something a bit more powerful in the 10000 RPM range. This is a serious post. Liquid nitrogen is 50 cents / L and paying to store my sperm is in the thousands. It seems like the rational thing to do.