Bluenoise

http://www.ncbi.nlm.nih.gov/ The place to start. Blast is very useful

Yes , but it's very unlikely that they'll be that simulair. It's very typical to use rabbits and horses for antibody production to each other or humans since they're all quite different. If you know the sequences of both the mouse and horse versions of this protein you can try to do a statistical analysis with bioinformatics tools. I doubt there would be any need to do this however, but it would only take a little while so I can't see any reason not to. got to get off the computer I'll catch you later.

Yes if you put a mouse protein into a horse it would almost certainly produce antibodies against that mouse protein.

I'm pretty sure there is, but you'd probably have to ask in the Physics forum. And I think it would be alot harder to setup. Using the ideal gas equation would be by far the easiest way to do this. (though not very accurate since steam is far from ideal) PV = nRT Change in pressure = (n x R x T1 / V1) - (n x R x T2 / V2) to give an estimate has to be the easiest way to do this.

No I don't think it would. I think the epitopes that the system would develope antibodies towards would probably be those that differ between the two species, so it should produce a unique enough antibody. One problem that I can see with this approach is that both these species are rodents and maybe so simulairly related that you might get no immune reaction at all. Using a horse would be far more effective I think, but that's probably not very plausible for research. Remember they used to use pig insulin in humans effectively.

1. You need to caculate the moles of h2so4. Use the corrent molar ratio (1:2 h2so4:KOH) to find how many moles of KOH you need. Then devide that my the concentration of your solution. 2. That depends on what you know or don't know. If you know nothing but the substance then you could do a titration or other analytical methods. 3. Yes H2SO4 + 2 KOH -> K2SO4 + 2 H20

Yeah try conjugating it by combining the coding regions of the mRNA. It may work, it may not. I'd do some research before investing the time into it though. Sorry for the double post.

No it wouldn't. You would need to inject it into something else. Like a rabbit for instance.

Well pharmaciticals promiss treatments. Stem cells promiss cures. So I assume if you're curing ppl there is a decrease in the need for pharmacuticals. Or at least that will be the instantanous effect. In the long run I could imagine a need direction in pharmacuticals to help with controlling integration of stem cells or bolstering their effetiveness. But definatley the most obvious outcome if stem cells prove very effective would probably be less need for drugs. Good topic btw

Never done this but I'd assume you'd combine the two coding regions from the mRNA, clone it express it and purify? Doing it chemically would probably very tricky. Have you tried just producing the antibody from the non-conjugated protein? You should still be able to get an immune response if it's placed in a forien host.

I second the femal models idea.

Ha ha I cheated!! but I swear I'll have it some day....

I'm sorry but I neither have the patients or the will to spend half an hour inventing a procedure. Giving someone suggestions and doing their homework for them are two completely different things. I hope I don't sound rude, I'm willing to answer any questions you have, but if the only reason you'll pass is because of the work of others well maybe you don't deserve to; redoing the course might be good for you.

Hmmm Human consumption? Any non-polar solvant.? Sounds like a planned extraction from something that I wont mention. If I'm guessing correctly that is. Butane is the solvent of choice for this purpose. It can be found pure in certain brands of butane fluid. Admittedly I've never attempted this and never will, but I'm sure this is plently of literature online describing this use, and including lists of good brands to use.

I see nothing that really stands out compared to other surverys. Asks about scienctific methods and then attempts to see if the person is taking more science courses.

Yeah it's true. Kinda stating the obvious. We care most about those that we are involved with the most. And you can't be involved with everyone....

Nice papers. btw do you know why to journal closed down? Or do you know of any simulair ones?

Sorry bought that budullewraagh you must have been writing that as I was submitting my edit.

Yes you can, and most litmus paper comes with a scale on the side. It's terribly inaccurate but it will give you some idea. Unfortunatley not all litmust paper is made equal, so you will need the specific scale for your paper. (Concentration of the chemical on the litmus paper can varry) *edit* Hmmm now that I think about this I can't specifically remember seing a scale on "blue" litmust paper. But I'm 100% possitive the multipurpose stuff we use has a scale on the side.

Nothing to do with genetics? That's rediculous. So you think that if we knocked out the genes responsible for your ability to feel certain emotions this couldn't have an effect on your personality? How about the fact that people with certain genetic dissorders and know to have much more agressive personalities? Either you have no idea what you're talking about, or you REALLY need to express yourself better. If you were to say "genetics are not one of the major factors" well I'd probably agree with you but saying it have NOTHING to do with genetics is laughable.

Um let me elaborate. Use a can with a screw top, after it's been crused place it in a graduated container and open it upside down, since there will still be a slight vacume in the can some water will get pulled in. Carefully screw it back on and measure the volume of water that got taken into the can. Use this to calculate the pressure difference between the crushed can and the external pressure. Subtract this from the external pressure and that gives you the pressure in the can after being crushed. Then you can use the total change in volume of the can to calculate the pressure change from right as it was cooled till it was opened. Subtract this from your pressure after crushing and you have the pressure once cooled. The pressure in the heated can will just be same as the external pressure. And if you use a pop can without a screw top well then it's an open system and the pressure will pretty much always be the same except for while it's being crushed. By the way I made this up off the top of my head. So if someone who knows better well knows better tell me so and I'll shut up.

Won't the pressure just be the same as the external pressure for before heating and after? I guess if you used a container with a screw top you could measure the change in volume of the can to determine the drop in pressure caused by the condensation of the steam. And just use Ideal gas law for simplicities sake... It wouldn't be terribly accurate but it would give you something. I don't think accuracy matters too much in a demonstration.

Hmmm I think you need to be more clear. I'm having trouble understanding what you're asking exactley. Yeild of what from what? Yeild is how much you get. % yeild is how much you get devided by some total, be it total protein content, total cell weight, expected yeild of the protein, yeild before purification, etc... I you don't know what this total is then you can't calculated the %yeild. Simple as that. Now you haven't said if the yeild is of a particular protein out of all the proteins, which sounds very likely since you seem to be purifying the proteins by gel exclusion. So then you must have recorded the total protein before putting them through the column or your out of luck. You mention 3 cavettes, two with simular values one very different. What did you do to get these? I'm sorry it's probably too late to help now anyways. Where the confusion really lies is that you're talking about a percentage. But you haven't mentioned what the total of the percentage is, which makes it a very arbitrary figure, nor have you given any data to suggest how to calculate anything but final mass/moles of protein. I wish I could help.

I'll be doing some research soon, and I'm in need of a REALLY good book that covers Biochemical and Molecular Biology techniqeus practices and interpretation of data. I've look around online a bit and am having lots of trouble deciding or finding lots of books that contain almost what I need but not enough. So anyone have any recomendations of a good one? I'd really like an oppinion before I decided to spend anymore money on books.

Acutally you can get the exactly opisite effect from having modular proteins if designed right. It increases the specificity alot of the time. Think of it as a key the more notches you have on the key the more specific it is to the lock it fits into. The trick is to "line up the notches" in such a manner so either they all bind or none bind. So maybe one may bind but because the one adjacent to it can't fit in it get's pushed away.