moosemanty

wow, thank you so much for the detailed response. it is very much appreciated. oh, and i'm an idiot. the concentration I needed for the thidiazuron was 2.5uM, not 0.5 . so does that mean i'd need 550.63 mg/l for a 1000x solution? Also I have a couple other ingredients. if you could check my math and answer my question below I would be extremely grateful. chemical- gibberellic acid molar mass- 346.37 g/mole concentration desired- 7 uM my answer- 2.43 grams a liter for a 1000x solution the final ingredient is indole-3-butyric acid. this one confuses me. the molecular mass is 203.24 g mol-1 what is the superscript -1? does that indicate that the atom has a negative ionic charge? if so does it change the way you would calculate it? if not then if I wanted a 2.5 uM concentrate would it come out to be 508.1mg per liter for a 1000x solution? oh and one final question, the recipe calls for "500mg l-1 of activated charcoal". what does the negative superscript 1 mean with that? Thank you again!

I am playing around with tissue culturing plants, and I am trying to mix a certain media mixture and it calls for 0.5 micromolar concentration of thidiazuron. I cannot find any info on how to measure that. can someone at least point me in the right direction on how to learn what I need to know to mix that concentration? I've never taken any chemistry classes so this leaves me a little confused.