peacefulspring

I've another question regarding DNA extraction from maize. So can anyone kindly teach me how to make fresh kernels to powder form? Is it by using liquid nitrogen so that the corn powder that we obtained would be to be extracted? And after which, can we simply use food mixer/ pestle/ mortar to 'grind' it to the powder form that we want? Thanks.

besides that, what would be the optimum voltage for 2% agarose gel and the duration for it? In addition, is the pore size of 2% agarose bigger than 1% agarose? Merged post follows: Consecutive posts mergedin addition to the previous post, the amplified products ranges form 100-600bp in size.. can anymore rly asap cos it's urgent!thanks..

Thus, if I choose to use Fluorometric Quantitation for my maize's DNA, would it be better? Merged post follows: Consecutive posts mergedbesides that, usually for negative control in detection for maize dna for instance, is the content of the negative control containing 1) only distilled water 2) everything (e.g. primer,polymerase & stuffs) except for DNA

But if I use Ethidium Bromide Fluorescence for the amplifiable DNA from maize (For the detection of GM maize experiement), is it alright too?

Hi, Can anyone kindly tell me how to quantify DNA other than using spectrophotometer/ gel electrophoresis. And how could we measure DNA using only 1microlitre? Thanks.

Hi, can anyone tell me how to dissolve EDTA with acid and alkaline with the concentration of the reagents also. Thanks.