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The question is cultivation of microalgae using BG11 medium. BG11 is a liquid medium for fresh microalgae. Analysis of lipid contents in microalgae requires too much volume of culture medium...

Dear all, Recently, I am performing a cultivation experiment that requires large volume of medium per sample. However, I am concerned about whether cultivation medium loss will affect experimental results or not. I'm not sure which strategy is better to reduce(minimize sampling loss) or increase(maximze data pont) sampling number. If you have any idea or related paper, please help me. Thank you.