Hello, I have to come up with a research project for university and I am planning to find possible binding partners of a specific signalling molecule in Drosophila. I have an antibody against the protein so I can co-precipitate the protein and its binding partners. Now my question, can I do the usal MALDI-TOF or would the data too hard to anaylse since there might be many mayn binding partners. Should I purify it before with a 2D gel to get results that I can easier interprete? Thanks in advance Tina
