girl322

I've tried to find it anywhere- but I can't. The companies sell only the protein - ectoine. But I am interested in the particular gene - ectA gene. So if I have a protein, do I create a cDNA library and then use primers to find a specific gene of interest?

Hi, I need a proper direction. I am conducting an independent experiment. The aim is to put an ectA gene into the yeast. It seems that I cannot get an ectABC operon from anywhere, however SigmaAldrich has ectoine for sale. So, I am having a protein - but I need to get a gene of interest. In the terms of experiment, how do I go from there? Do I need to construct a cDNA? Regards, I.