LobsterFarmer

Ive been using a custom made mastermix that for some reason results in a qPCR curve that has a baseline lift. I have attached a crude drawing of what the graph looks like. Due to the baselift lift the CP values are really early, as early as cycle 15. Playing with the initial denaturation temp, denaturation and annealing steps didnt solve the problem. Any help would be greatly appreciated. Thanks