DK79

i will read up on it. I know i have seem this type of curve in the past but have not been able to explain its nature. Thank you!!

I have performed a binding experiment using streptavidin agarose beads and a biotynilated molecule ranging from high to low concentration. The molecule exhibits close to 100% capture at low concentration as is expected then gradually drops off. The drop -off is more extreme at higher concentrations. If the beads become saturated past a certain concentration, should the drop off be at a steady rate? Why is there seemingly a slow drop-ff initially then a fast one (it should be noted that the % captured is measured by fluorescence) Binding curve is below. The beads are Thermo Scientific 7.5% Agarose slurry. Thanks!

I have performed a binding experiment using a biotynilated molecule ranging from high to low concentration. The molecule exhibits 100% binding at low concentration (as expected) then gradually drops off. The drop -off is more extreme at higher concentrations. If the beads become saturated past a certain concentration shouldnt the curve drop off quicker and at a steady rate? (it should be noted that the % captured is measured by fluorescence) Binding curve is below. The beads are GE's Sepharose Mag beads cat # 28-9857-38 Thanks!