Shinra123

Thanks for the reply. I just have a couple of follow up questions. What do you mean by the double required volume (I ul ?) and could you elaborate on how to do the 1:2 dilutions to get the desired range which is 0-800ng/ml so 0, 100, 200 etc?

Hey, Disclaimer: This is my first post so I hope this is in the right section. I'm doing an experiment on different anticoagulants by comparing how they effect different specific clotting factors (measured via a factor activity assay). I have 2.5 mg of apixaban (in tablet form) and I wanted to make a stock solution out of it and then use 5 different final concentrations ranging from say 0 - 800 ng/ml i.e. 50ng/ml ... 150 ng/ml etc and use these solutions to spike normal plasma samples. Then I will perform the activity assays.I have only have a limited supply of the anticoagulants in tablet forms and was wondering how I could make the final concentrations i.e. the stock then the exact concentrations I need. I'm dissolving the drugs in distilled water and not DMSOAny help is appreciated