DavidG

OK, I have to admit a college student's question got me started down this same path, and I reach very different conclusions from the response given by CharonY. Its possible that progress in the years since the question was first posted have significantly lowered the difficulty, but I DO see this as an excellent DIY project. CharonY's comment that the 'construction of the vector is relatively trivial but that the equipment is prohibitively expensive' reminds me of the early days of making a color printout: while the equipment required to produce a color printout was expensive, someone how HAS the right equipment would be open to making a printout on your behalf. Which had me thinking that anyone WITH the right equipment might be open to the earning a little on the side as a service to those who don't have the equipment. And with a little searching, I learned that: yep, not only have folks made these services available but that its gone mainstream. You can order exactly what you need, ready made and ready to go, from places like: https://www.thermofisher.com/us/en/home/life-science/cell-analysis/fluorophores/green-fluorescent-protein.html (I might mention that for me growing up, getting that latest Fisher Scientific catalog was always a highlight of my year!) With a little more digging around, I found this outstanding publication by Scott that I plan to dive into during the upcoming holiday break: 'Generation of axolotl hematopoietic chimeras', http://www.jbmethods.org/jbm/article/view/43/34 Citation: Lopez D, Scott EW. Generation of axolotl hematopoietic chimeras. J Biol Methods 2015;2(1):e12. doi: 10.14440/jbm.2015.43 So DaveDendro: did you give up? Did you make progress? Did you end up pursuing your line of inquiry? I hope you did - and would love an update! Sincerely, DavidG