In the most accepted hypothesis of oxidative phosphorylation, P . Mitchel gave an account on d transport of electrons in d electron transport chain on d inner membrane of d mitochondria , d generation of electrochemical gradient that drives d synthesis of ATP, the presence of d enzyme ATPase (a membrane bound protein in the inner membrane of the mitochondria) and hence the generation of ATP.
In Rackers experiment, the membrane was distorted to isolate d fragments of d membrane from d protein which was d F1 Fo ATPase. On his conclusion, electron transport was possible on d fragments, witout phosphorylation, and there was phosphorylation by d protein fraction and then,d recontruction gives oxidative phosphorylation. My question is this:
- Where was d energy derived from in Rackers experiment that caused d phosphorylation of ADP since d membrane is damaged? Cos an electrochemical gradient cannot be generated in d fragmented mitochondria.