Dear Sir or Madam,
I do not really understand how to design a primer for a given DNA sequence. For example I have given this DNA sequence
841 aaaccagagc cggccagcca gtgaaacagc caccgtggag gggggacggc gaaaa***atg***aa
901 atccaaccaa gagcggagca acgaatgcct gcctcccaag aaacgtgaga tccccgccac
...
3241 catcgaaggc cgatctaacg tgggcaag***ta g***agaccttgc gggcagcgga ggcccggggc
3301 ctccttttac tgtctgtatc cagattactg tactgtaggc taagtaacac agtatttaca
Now I want to design Primers for a PCR to amplify the region marked by the start and stop codon. How do I do that? What do I need to concern? Can you recommand any literature where there is described step-by-step what to do in this situation? And how can I add sequences for EcoRI or BamHI.
I really need your help and I would be glad if someone could explain me the basic steps...
Best wishes,
chesspuma99
