plk21

I really need someone to start me off with my practical questions. 1. Buffer will be 100ml of 0.25 M Tris buffer with a ph of 8.0. Write out the chemical equation for this buffer. Identify which species is the conjugate acid and conjugate base. 2. For this buffer, calculate the ratio of the base to acid using the Henderson-Hasselbach equation. I don't really know where to begin to be honest. Any help/ info would be so appreciated!