beachbum

yes thats true that yeast wouldn't have anything to gain from it but it could be just a stimulated response. the zymosan thing is pretty interesting, but I really can't risk using LPS if there isn't published support. I've checked chemical company catalogues and nothing has come up but by any chance, do you know of any NOS inducing drugs?

thanks but i've checked google scholar and the University of florida library database but nothing has come up about yeast being sensitive to LPS addition.

I really like PNAS (proceedings of the national academy of sciences), great research. As for medical sciences, the lancet is pretty awesome too, except one issue only has about 3-4 articles so its not really worth the money but its a prestigious journal and the research is very good too.

oooh thanks herpguy

well I guess its pollen season again and my sister is having this huge allergy attack. Do any of you guys know some good home remedies or foods/herbs for allergy relief b/c she is EXTREMELY sensitive to medicines. Or maybe the medicine with midlest/least side-effects? thanks.

Hi, I am trying to add a chemical to some yeast cells but I found out that this chemical can't penetrate through the yeast cell wall. Then I thought of adding a lysate buffer to lyse the cell, therefore the cell wall wouldn't be a problem. But does lysing the cells prevent it from functioning and being "normal". Thanks.

is microsoft going to send you alotta product info and advertising stuff though?

does anyone know of a nonspecific nitric oxide or nitric oxide synthase drug inducer? not LPS or any cytokines. Thankyou.

can you give us a hint about the book?

Does anyone know of a nonspecific (if that exists) caspase inhibitor or inhibitors of caspase-3 and/or caspase-8? in addition, what assay technique would you need to measure the activation/activity of a caspase?

Hi, i am doing a project on yeast. lipopolysaccharide is usually used in mammalian cells to induce NOS expression but does anyone have literature that has demonstrated yeast sensitivity to LPS? secondly, how does someone come upon identifiying a downstream regulator to a process? is it mainly just reviewing literature like determining an inducer/inhibitor for a process or more different? Thankyou.

i created a birthday card on paint and I have some flower shaped things on the top corner of the page but I want to move them to the bottom corner of the page, is there a way I can "move" them without erasing the image and drawing it again since these are very tedious pictures to draw. are there other programs like adobe photoshop that can do this? Thankyou.

When I think of a computer, I think play, play, play

I am conducting a project where I grew different dilutions (1:10-1:10^6) of 6 different strains of cells in 4 different drugs to induce cell death and grew them on petri dishes. I am not sure if there are different types of siginificance tests but what type of significance test would I need to use in this project? What other types of statistical analysis would be needed? Help is apreciated, thankyou.

i downloaded a NIH software called ImageJ to analyze the area of growth for some cell cultures which were photographed and put into a ppt. document. however, when I try to upload/open it in the program, a window popps up that says "plugin not found: ZVI_Reade". I checked the available plugins but ZVI_Reade doesn't exist. What does this mean and do I need to download a plugin? Thankyou.

when researchers test different symptoms or processes in the human body they use different cells for experimentation. how do you know how to pick the right cell line?

I have been trying to figure out these challenge problems in my Chemistry 30 class for the past week but have found no luck. here goes: molecules of NH3 are polar but molecules of BF3 aren't. SbCl3 has a measurable dipole, while SbCl5 doesn't. Explain both problems by means of atomic and molecular structures and also regarding principles of bonding.

if anorexics don't eat anything and become really thin, then why do bulimics "maintain" body weight instead of end up the same as anorexicx when they purge out everything they've just eaten? doesn't purging pretty much take out all the calories that they once consumed.

do you think that working on signal transduction for 9th graders is a bad idea to enter a science project? got an awesome project on mind but the science department head won't allow it because I haven't taken previous biotechnological lab courses.

i know alotta really talented people that went to stanford to study neuroscience and plant biology so i guess that counts as biology. Stanford's also ranked higher generally but I haven't really checked out the other schools yet. btw. does anyone know which ivy or semi-ivy(duke, stanford etc..) has the best psychology program for psychology major?

yea but is there an easier calculation? like finding LCM for example (although thats not the way to do it) but something like that? or else it takes a while to figure out the coefficients especially for more complicated molecules.

our teacher tells us to just keep on adding coefficients and dividing or multiplying it by 2 until its balanced and it takes FOREVER to balance an equation.

Has anyone attended it? What did you think?

yea i've always thought of this if everyone had the same capacity for intelligence. maybe they do for genetic and interest reasons and the ability to understand some things more than others. but then how can you explain people that aren't good at anything or people that are good at everything (like da vinci for example)?

especially the thing when it refers to carbon all the time w./ the 1/12 amu thing. can anyone explain more thoroughly?