muntedkowhai

actually, echanachea has had some bad publicity. in a test of 200 human subjects. 100 were given recomended doses of echanachea for half a year. the other 100 didnt take supplements and were all given the same dose of influenza. 99% of them came down with the flu. So maybe these herbs arent as immune boosting as the supplement companies want you to think. and plants do have some sort of immune response which some papers have seen also happening in humans. they assume that the system seen in plants are probably the first kind of immune system known to living things. it is RNAi. There has been tremendous research done on RNAi .

there has been some research done on H5N1. ingenius research using an eight plasmid system that will allow vaccines to be grown outside of chicken eggs. the reason that there is no vaccine for H5N1 is because it can not grow in the chicken eggs without killing the embryo too fast. it only lasts about 2 days or so. (This is due to the lethal few amino acids observed in the HA region of its genome) The alteration of this amino acids have proven to attenuate the viruse in mice and if more money is poured into the region, it will be possible not only to have a vaccine for H5N1 but ease the pain of influenza shortages. (and have back stocks of possible antigenic changes) if you are interested in reading on this paper, Eight plasmid system for rapid generation of influenza virus vaccines. Vaccine.20.2002.3165-3170

thanks guys, i think i'll just re precipitate the dna construct, if theres any of it left. then run the gel on it again. well i'll just precipitate it first and then see if theres even anything there.

well this was a kit you see, its less cumbersome then working with phenol, as my co worker said. but alas, i lost the dna constructs in that eppie, so theres really nothing i can do now. :<

So I'm bulking up my DNA constructs and I ran them on the gel today. I saw RNA in one of my lanes. my co worker told me i could use phenol and some washes to get rid of RNA or use a kit she had gotten from a biotech company. i chose the latter because, hey its phenol. i went thru all the steps but after a few washes, i did not see any pellet and when i put it thru the nanodrop. it came out with negative nanograms per microliter. ok not good. so where the hell in the washing steps could have gone wrong? i was careful in the washing, i pippett only the SN and nothing more. the only thing i can think of is, i left the solution in over 2 minutes the suggested time. the solution was the kits' ligase wash. so i dontknow if that would have done it. i mean before i did this, i had 1050 ng/ul of dna and rna in the sample. any suggestions?

e.coli is right when we're talking about gram stainning, cell walls should not be mentioned. (i have learnt this the hard way, thanks to an anal retentive microbio advisor) to truly understand the mechanisms of gram stainning, we should start using the correct terminology of the structures involved in stainning.