I imagine similar to known ones? As I understand it (and perhaps inaccurately), keratinases are different in just about every fungus that has them. Because there has been a lot of work looking at keratin degradation in Aspergillus fumigatus, I figured I could potentially use it as a reference even though the fungi of interest in are reptile pathogens.
Okay. What about using a reference genome of a closely related species to create a probe looking for similar enzymes? Is that getting more into qPCR?
Sorry for these ongoing questions, I do appreciate your answers though!
Hello,
I'm trying to find a method to extract enzymes from filamentous fungi. I'm not a molecular biologist by any means so I have no idea which methods does what.
I came across SDS-PAGE, which as I understand separates enzymes based on weight?
To give some context, some of these fungi don't have annotated genomes. So basically what I'm asking is SDS-PAGE a good method to identify which enzymes exist in a fungal sample?
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