Molbio_learning

Thank you for the reply. Yes, I want to get biotinylated product directly during synthesis, how do I order such primer?

Hello everyone, this is my first post and I am hoping it is the right place to ask. I just recently started cloning, PCR design and similar. I have very basic question and would like to get an answer step by step if possible, using an example 🙏🏻 So I have a pool of oligos (with short stretch of the same sequence at 5’ and 3’ so I can do PCR with it, for example to introduce nuclease sites). My question is, how do I design a primer for PCR to introduce biotin site and let’s say one of any restriction enzymes?? I hope I provided enough of info, I need only one primer. thanks a lot!!! 🤓